OpenCloning Documentation

OpenCloning is an Open-Source web application to plan and document cloning. Users can:

• 📡 Import plasmid sequences from AddGene and gene sequences from NCBI.
• 📁 Load their own sequence files.
• 🧬 Plan cloning and design primers using common techniques (Gibson, golden gate, gateway, etc.).
• 🦠 Plan strain and cell line engineering via CRISPR and homologous recombination, with use-cases not supported by SnapGene or Benchling.
• 🤖 Automate repetitive cloning and primer design using scripts or web forms.
• 💾 Download final constructs as GenBank or FASTA files.
• 📜 Archive the entire cloning history in an Open format and load it later.
• 🛠️ Create reusable cloning templates for cloning kits.

Getting started

The best way to get a feeling of what OpenCloning can do is to watch this demo video (slightly outdated, but the core functionality is the same). For a more detailed overview, check the other sections of this documentation in the order they appear on the left sidebar.